PCR in real time. 11th ed. Ribrikov D.V., Samatov G.A., Trofimov D.Yu.

Various options and features of equipment for the PCR “In Real Time” are considered, recommendations for the choice of the amplifier are given. The features of fluorescent registration systems of DNA accumulation are dismantled. The key factors are considered that determine the choice of the sequence of oligonucleotides and the parameters of the amplification programs. Attention was paid to the preparation of samples and the features of the analysis of the data obtained, which is necessary to obtain the most reliable results. Certain chapters are devoted to the use of PCR “in real time” to solve various tasks: determining the level of representation of transcriptions, viral load, nucleotide polymorphism, the relative content of nucleic acids using the example of GMOs. , as well as for teachers and students specializing in the field of molecular biology.